Nucleic acid analysis on electrowetting-based digital microfluidics

doi:10.1016/j.trac.2022.116826

UM > INSTITUTE OF MICROELECTRONICS

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	Nucleic acid analysis on electrowetting-based digital microfluidics
	Shen, Ren 1; Lv, A'man 1; Yi, Shuhong 2; Wang, Ping 3; Mak, Pui In 1,4; Martins, Rui P.1,4,5 ; Jia, Yanwei1,4,6
	2023
Source Publication	TrAC - Trends in Analytical Chemistry
ISSN	0165-9936
Volume	158
Abstract	Since the disclosure of DNA/RNA as the genetic material, nucleic acid analysis has been an indispensable category in molecular diagnosis, disease prediction, prognosis, and fundamental biological research. Among various platforms for the analysis of DNA/RNA, electrowetting-based digital microfluidics (DMF) has emerged as a promising tool. Using electrical signals to manipulate individual droplets, DMF devices could be packaged into compact automatic systems for multiplex analytical assays in a miniaturized format, especially suitable for point-of-care applications. Herein, we review basic technical principles that support nucleic acid analysis on DMF platforms and recent advances in applications regarding nucleic acid extraction, amplification, and further analysis such as sequencing on DMF. We also discuss the commercialization status of related technology and applications, alongside our opinions on the future development of digital microfluidics for nucleic acid analysis.
Keyword	Digital microfluidics Electrowetting Nucleic acid amplification Point-of-care Sequencing
DOI	10.1016/j.trac.2022.116826
URL	View the original
Language	英語English
Scopus ID	2-s2.0-85142200251
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science View Full-Text via Scopus
Citation statistics
Document Type	Journal article
Collection	INSTITUTE OF MICROELECTRONICS DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
Affiliation	1.State-Key Laboratory of Analog and Mixed-Signal VLSI, Institute of Microelectronics, University of Macau, Macau, China 2.Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China 3.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China 4.Faculty of Science and Technology – ECE, University of Macau, Macau, China 5.On Leave from Instituto Superior Técnico, Universidade de Lisboa, Portugal 6.MoE Frontiers Science Center for Precision Oncology, University of Macau, Macau, China
First Author Affilication	University of Macau
Recommended Citation GB/T 7714	Shen, Ren,Lv, A'man,Yi, Shuhong,et al. Nucleic acid analysis on electrowetting-based digital microfluidics[J]. TrAC - Trends in Analytical Chemistry, 2023, 158.
APA	Shen, Ren., Lv, A'man., Yi, Shuhong., Wang, Ping., Mak, Pui In., Martins, Rui P.., & Jia, Yanwei (2023). Nucleic acid analysis on electrowetting-based digital microfluidics. TrAC - Trends in Analytical Chemistry, 158.
MLA	Shen, Ren,et al."Nucleic acid analysis on electrowetting-based digital microfluidics".TrAC - Trends in Analytical Chemistry 158(2023).

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