Metabolism and gene expression are tightly interconnected processes. The orchestration of global gene expression involves complex networks of transcriptional regulators, including kinases, chromatin modifiers, and transcription factors. The functions of these regulators are influenced by the metabolic state of the cell, and in turn, their effects on global gene expression shape cellular metabolism. Thus, studying the functions and regulation of transcriptional regulators can provide valuable insights into metabolism. In our laboratory, we employ a Functional Genomics approach to investigate the genome-wide targets and transcriptional effects of various transcriptional regulators involved in metabolism. By deciphering the regulatory networks and transcriptional responses associated with metabolic processes, we aim to gain a broader understanding of cellular metabolism and its regulation and develop new strategies for manipulating metabolic processes in fungi.