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Patent Number202211554310.9
Status申請中 Pending
一种检测中药中噻虫嗪含量的免疫分析方法及免疫试剂盒
Year Issued2022
2022-12
Application NumberCN202211554310.9
Application Date2022-12-06
李鵬; 崔錫平; 魏金超; 盧健; 王勝鵬; 王一濤
Country中國
Subtype发明专利 Invention
Abstract

本发明公开了一种检测中药中噻虫嗪含量的免疫分析方法,其包括向包被有噻虫嗪完全抗原的固相载体中加入生物素标记的抗噻虫嗪多克隆抗体、样品溶液,反应后再加入链霉亲和素标记的辣根过氧化物酶溶液,再次反应后分别依次加入底物液和终止液,检测吸光值并计算得到噻虫嗪含量。本发明通过样品与噻虫嗪完全抗原竞争结合生物素标记的抗噻虫嗪多克隆抗体,在生物素-链霉亲和素的作用下增强信号,实现对噻虫嗪含量的检测。

Document TypePatent
CollectionInstitute of Chinese Medical Sciences
Affiliation澳门大学
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
李鵬,崔錫平,魏金超,等. 一种检测中药中噻虫嗪含量的免疫分析方法及免疫试剂盒. 202211554310.9[P]. 2022-12-01.
APA 李鵬., 崔錫平., 魏金超., 盧健., 王勝鵬., & 王一濤 (2022-12-06). 一种检测中药中噻虫嗪含量的免疫分析方法及免疫试剂盒.
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