Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

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	Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.
	LI, P; Wang, L.; Di, L.
	2019-08-02
Source Publication	J Proteome Res
ISSN	1535-3893
Pages	2987-2998
Abstract	Protein-protein interactions (PPIs) are indispensable for the dynamic assembly of multiprotein complexes that are central players of nearly all of the intracellular biological processes, such as signaling pathways, metabolic pathways, formation of intracellular organelles, establishment of cytoplasmic skeletons, etc. Numerous approaches have been invented to study PPIs both in vivo and in vitro, including the protein-fragment complementation assay (PCA), which is a widely applied technology to study PPIs and biomolecular interactions. PCA is a technology based on the expression of the bait and prey proteins in fusion with two complementary reporter protein fragments, respectively, that will reassemble when in close proximity. The reporter protein can be the enzymes or fluorescent proteins. Recovery of the enzymatic activity or fluorescent signal can be the indicator of PPI between the bait and prey proteins. Significant effort has been invested in developing many derivatives of PCA, along with various applications, in order to address specific questions. Therefore, a prompt review of these applications is important. In this review, we will categorize these applications according to the scenarios that the PCAs were applied and expect to provide a reference guideline for the future selection of PCA methods in solving a specific problem.
Keyword	BiFC biochemical network biomolecular interactions protein-fragment complementation assays split enzymes
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	45222
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Di, L.
Recommended Citation GB/T 7714	LI, P,Wang, L.,Di, L.. Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.[J]. J Proteome Res, 2019, 2987-2998.
APA	LI, P., Wang, L.., & Di, L. (2019). Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.. J Proteome Res, 2987-2998.
MLA	LI, P,et al."Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.".J Proteome Res (2019):2987-2998.

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