N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

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	N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies
	Li, D; Liu, J; Zhang, L; Xu, T; Chen, J; Wang, L; Zhao, Q
	2015-12-21
Source Publication	Virologica Sinica
ISSN	1674-0769
Pages	449-456
Abstract	The Human immunodeficiency virus type 1 (HIV-1) gp41 membrane proximal external region (MPER) is targeted by broadly neutralizing antibodies (e.g. 2F5, 4E10, Z13e and m66.6), which makes this region a promising target for vaccine design. One strategy to elicit neutralizing antibodies against the MPER epitope is to design peptide immunogens mimicking neutralization structures. To probe 2F5-like neutralizing antibodies, two yeast-displayed antibody libraries from peripheral blood mononuclear cells from a HIV-1 patient were screened against the 2F5 epitope peptide SP62. Two 2F5-like antibodies were identified that specifically recognized SP62. However, these antibodies only weakly neutralized HIV-1 primary isolates. The epitopes recognized by these two 2F5-like antibodies include not only the 2F5 epitope (amino acids (aa) 662–667 in the MPER) but also several other residues (aa 652–655) locating at the N-terminus in SP62. Experimental results suggest that residues of SP62 adjacent to the 2F5 epitope influence the response of broadly neutralizing 2F5-like antibodies in vaccination. Our findings may aid the design of vaccine immunogens and development of therapeutics against HIV-1 infection.
Keyword	HIV-1 MPER 2F5 neutralizing antibody yeast display
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	19381
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Wang, L; Zhao, Q
Recommended Citation GB/T 7714	Li, D,Liu, J,Zhang, L,et al. N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies[J]. Virologica Sinica, 2015, 449-456.
APA	Li, D., Liu, J., Zhang, L., Xu, T., Chen, J., Wang, L., & Zhao, Q (2015). N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies. Virologica Sinica, 449-456.
MLA	Li, D,et al."N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies".Virologica Sinica (2015):449-456.

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