| Status | 已發表Published |
| 大容量人源 Fab 噬菌体展示抗体库的构建及抗 CD276 抗体筛选 | |
| Alternative Title | Construction of a large human Fab phage display antibodies library and screening of phage antibodies against CD276 |
Liu, J.; Chen , J.; Xu, T.; Wang, L.; Zhao, Q.
| |
| 2014-08-01 | |
| Source Publication | 中国医药生物技术
 |
| ISSN | 1673-713X |
| Pages | 173-277 |
| Abstract | Objective To construct a large human Fab antibodies library and to screen and identify the anti-CD276 phage antibodies from the library. Methods Total RNA was extracted from peripheral blood lymphocytes of 10 healthy donors, and the Fab antibody genes were amplified by RT-PCR. The amplification products were sequentially cloned into phage vector pCANTAB5E to construct a humanFab phage antibodies library. Antibodies against CD276 were screened using immobilized antigen. After three rounds of screening, 96 randomly selected clones were identified by phage-ELISA to select specific ones with high affinity for CD276. Results A large human Fab phage-display library consisting of 5 × 1010 members was successfully constructed. From the phage library, we obtained eleven positive clones which had specificity and binding reactivity towards CD276. By sequencing, all of the 11 clones had the same sequences, and their specificity was confirmed by ELISA. Conclusion We successfully constructed a large human Fab phage antibodies library and isolated the specific human anti-CD276 Fab antibodies, which provided an experimental foundation in future study and application. |
| Keyword | Immunoglobulin Fab fragments Phage display antibody library CD276 |
| URL | View the original |
| Language | 其他語言Others |
| The Source to Article | PB_Publication |
| PUB ID | 55352 |
| Document Type | Journal article |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES |
| Corresponding Author | Zhao, Q. |
| Recommended Citation GB/T 7714 | Liu, J.,Chen , J.,Xu, T.,等. 大容量人源 Fab 噬菌体展示抗体库的构建及抗 CD276 抗体筛选[J]. 中国医药生物技术, 2014, 173-277. |
| APA | Liu, J.., Chen , J.., Xu, T.., Wang, L.., & Zhao, Q. (2014). 大容量人源 Fab 噬菌体展示抗体库的构建及抗 CD276 抗体筛选. 中国医药生物技术, 173-277. |
| MLA | Liu, J.,et al."大容量人源 Fab 噬菌体展示抗体库的构建及抗 CD276 抗体筛选".中国医药生物技术 (2014):173-277. |
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