To address the growing resistance of Gram-negative bacteria and enhance the clinical application potential of antimicrobial peptides. In this study, we combined two α-helical antimicrobial peptides K (WRKWRKWRKWRK-NH) or K (WRKWRKWRKWRKWRK-NH) with four traditional antibiotics (gentamicin, rifampicin, ciprofloxacin, and imipenem). Then we explored their potential and mechanism in inhibiting the generation of bacterial resistance. After adding K or K, the tendency to produce resistant bacteria from traditional antibiotics declined significantly. And the obtained antibiotics-resistant bacteria were further re-sensitive to their corresponding traditional antibiotics with the addition of K or K. Mostly, the combination showed synergistic or additive antibacterial effects on both the standard strains and the obtained antibiotics-resistant bacteria, with a much better effect on the latter. Chemical sensitization and the outer membrane permeability experiments demonstrated that K and K may influence the drug efflux of the tested bacteria, while at the same time improving the outer membrane permeability of the obtained antibiotics-resistant bacteria to traditional antibiotics by acting as a film breaker. They ultimately eliminated the generation of drug resistance. These results provided an effort that could break Gram-negative bacteria's resistance.