| Status | 已發表Published |
| Hydrogen peroxide induced the activation of JNK/P38MAPK pathways in retinal neuronal cells and promoted the survival of these cells | |
Zheng, W. 
| |
| 2015-06-01 | |
| Source Publication | Proceeding of 7th conference of Guangzhou Society of Pharmacology
 |
| Pages | 113-114 |
| Publication Place | Guangzhou |
| Publisher | Guangdong Societ of Pharmacology |
| Abstract | Hydrogen peroxide induced the activation of JNK/P38MAPK pathways in retinal neuronal cells and promoted the survival of these cells Lang Zhang, Gao Yan, Philip Lazarovici and Wenhua Zheng* State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center, Sun Yat-Sen University and Faculty of Health Science, University of Macau, Macau, China. *corresponding author. Abstract Purpose: To study the effect of hydrogen peroxide (H2O2) on the apoptosis of retinal neuronal RGC-5 cells induced by serum deprivation and the underlying mechanisms. Methods: RGC-5 cells deprived of serum were used as a toxicity cellular model. The effect of H2O2 on the viability and apoptosis of RGC-5 cells was evaluated by MTT and Hoechst staining assays. The activation of kinases was determined by Western blotting with specific phospho-antibodies. Kinases or proteins involved were investigated by the application of kinase specific inhibitors or specific siRNA. Result: Serum deprivation induced apoptosis of RGC-5 cells. Low concentrations of H2O2 (0-30 µM) blocked serum deprivation induced apoptosis of RGC-5 cells while a toxic effect was observed at higher concentration of H2O2 (>60 µM). Western blotting showed that 30 µM H2O2 significantly stimulated the activation of JNK and P38 MAPK while it had no effect on the activation of Akt, ERK1/2 and other survival kinases. Application of JNK or P38 MAPK specific inhibitors inhibited the protective effect of H2O2 although JNK inhibitors were more potent than inhibitors of P38 MAPK. Moreover, 30 µM H2O2 induced the phosphorylation of c-Jun time-dependently. Blockade of the phosphorylation of c-Jun induced by H2O2 or down-regulated the expression of c-Jun by siRNA attenuated the protective effect of H2O2 on RGC-5 cells. Conclusion: The JNK/c-Jun and P38MAPK pathways play important roles in the protective effect of low concentrations of H2O2 on serum deprivation-induced injury of RGC-5 cells. Supported by National Natural Science Fund of China (31371088), Funding from Guangdong Science and Technology Department (No. 2011B050200005). Funding from State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center and SRG at University of Macau. |
| Keyword | Hydrogen peroxide JNK/P38MAPK retinal neuronal cells survival |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 15374 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF PHARMACEUTICAL SCIENCES |
| Corresponding Author | Zheng, W. |
| Recommended Citation GB/T 7714 | Zheng, W.. Hydrogen peroxide induced the activation of JNK/P38MAPK pathways in retinal neuronal cells and promoted the survival of these cells[C], Guangzhou:Guangdong Societ of Pharmacology, 2015, 113-114. |
| APA | Zheng, W..(2015). Hydrogen peroxide induced the activation of JNK/P38MAPK pathways in retinal neuronal cells and promoted the survival of these cells. Proceeding of 7th conference of Guangzhou Society of Pharmacology, 113-114. |
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