| Status | 已發表Published |
| Artemisinin Protected PC12 cells and Primary Cortical Cultured Neurons against Sodium nitroprusside-induce oxidative injure cia activation of ERK | |
Zheng, W. 
| |
| 2015-11-04 | |
| Source Publication | The 9th Pong Ding Yuen International Symposium on Traditional Chinese Medicine
 |
| Abstract | Objective Nitric oxide(NO) is implicated in brain injury and early neurological deterioration in acute ischemic stroke. In present study, we investigated the protective effects of Artemisinin against sodium nitroprusside(SNP)-induced oxidative injury in PC12 cells and cortical neurons as well as exploring its underlying mechanism. Methods Cells were treated with SNP to create a neuronal injury model. The protective effect of Artemisinin was evaluated by pre-treatment of PC12 cells with Artemisinin before exposure to SNP. The cell viability and nuclear morphology were evaluated using MTT assay or Hoechst staining respectively. Activation of protein kinase B (Akt) and extracellular regulated protein kinase (ERK) was determined by Western blotting analysis. The kinase pathway involved in the effect of Artemisinin was studied by kinase inhibitors. Results Artemisinin significantly attenuated SNP-induced oxidative injury in PC12 cells and primary cortical cultured neurons by stimulating the activation of ERK but that of AKT. Moreover, inhibition of ERK with the ERK pathway inhibitor, PD98059 blocked the protective effect of Artemisinin while the PI3K inhibitor, LY294002 had no effect. Acknowledgement: Partly supported by the organizers of the conference. |
| Keyword | Nitric oxide brain injury early neurological deterioration Artemisinin ERK signaling pathway |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 19191 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF PHARMACEUTICAL SCIENCES |
| Corresponding Author | Zheng, W. |
| Recommended Citation GB/T 7714 | Zheng, W.. Artemisinin Protected PC12 cells and Primary Cortical Cultured Neurons against Sodium nitroprusside-induce oxidative injure cia activation of ERK[C], 2015. |
| APA | Zheng, W..(2015). Artemisinin Protected PC12 cells and Primary Cortical Cultured Neurons against Sodium nitroprusside-induce oxidative injure cia activation of ERK. The 9th Pong Ding Yuen International Symposium on Traditional Chinese Medicine. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment