| Status | 已發表Published |
| Protective effect of metformin against hydrogen peroxide induced oxidative damage in RPE cells by enhancing autophagy through activation of AMPK pathway Abstract | |
Zheng, W. 
| |
| 2020-05-19 | |
| Source Publication | 4th Symposium on Biomedical Sciences for Students, Postdoctoral Fellows and Research Assistants 2020
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| Abstract | Age-related macular degeneration (AMD) is a leading cause of blindness with very limited effective treatment. Although the pathogenesis of this disease is complex and not fully understood, the oxidative damage caused by excessive ROS in RPE, has been considered as a major cause. Autophagy is essential for the degradation of cellular components damaged by ROS and its dysregulation has been implicated in AMD pathogenesis. Therefore, strategies aiming to boost autophagy could be effective in protecting RPE cells from oxidative damage. Metformin is the first-line anti-type 2 diabetes drug and has been reported to stimulate autophagy in many tissues. We therefore hypothesized that metformin may be able to protect RPE cells against H2O2-induced oxidative damage by autophagy activation. In the present study, we found that metformin attenuated H2O2-induced cell viability loss, apoptosis, elevated ROS levels as well as the collapse of the mitochondria membrane potential in D407 cells. Autophagy was stimulated by metformin, and inhibition of autophagy by 3-Methyladenine and chloroquine or knockdown of Beclin1 and LC3B blocked the protective effects of metformin. In addition, we showed that metformin could activate the AMPK pathway, whereas both pharmacological and genetic inhibition of AMPK blocked the autophagy-stimulating and protective effects of metformin. Metformin conferred a similar protection against H2O2-induced oxidative damage in primary cultured human RPE cells. Taken together, these results demonstrate that metformin could protect RPE cells from H2O2-induced oxidative damage by stimulating autophagy via the activation of AMPK |
| Keyword | AMD hydrogen peroxide-induce oxidative damage AMPK Pathway |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 51490 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF PHARMACEUTICAL SCIENCES |
| Corresponding Author | Zheng, W. |
| Recommended Citation GB/T 7714 | Zheng, W.. Protective effect of metformin against hydrogen peroxide induced oxidative damage in RPE cells by enhancing autophagy through activation of AMPK pathway Abstract[C], 2020. |
| APA | Zheng, W..(2020). Protective effect of metformin against hydrogen peroxide induced oxidative damage in RPE cells by enhancing autophagy through activation of AMPK pathway Abstract. 4th Symposium on Biomedical Sciences for Students, Postdoctoral Fellows and Research Assistants 2020. |
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