Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs.

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Status	已發表Published
	Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs.
	He, M.; Qin, H.; Poon, T. C. W.; Sze, S. C.; Ding, X.; Co, N. N.; Ngai, S. M.; Chan, T. F.; Wong, N.
	2015-09-01
Source Publication	Carcinogenesis.
ISSN	0143-3334
Pages	1008-1018
Abstract	Exosomes are increasingly recognized as important mediators of cell-cell communication in cancer progression through the horizontal transfer of RNAs and proteins to neighboring or distant cells. Hepatocellular carcinoma (HCC) is a highly malignant cancer, whose metastasis is largely influenced by the tumor microenvironment. The possible role of exosomes in the interactions between HCC tumor cell and its surrounding hepatic milieu are however largely unknown. In this study, we comprehensively characterized the exosomal RNA and proteome contents derived from three HCC cell lines (HKCI-C3, HKCI-8 and MHCC97L) and an immortalized hepatocyte line (MIHA) using Ion Torrent sequencing and mass spectrometry, respectively. RNA deep sequencing and proteomic analysis revealed exosomes derived from metastatic HCC cell lines carried a large number of protumorigenic RNAs and proteins, such as MET protooncogene, S100 family members and the caveolins. Of interest, we found that exosomes from motile HCC cell lines could significantly enhance the migratory and invasive abilities of non-motile MIHA cell. We further demonstrated that uptake of these shuttled molecules could trigger PI3K/AKT and MAPK signaling pathways in MIHA with increased secretion of active MMP-2 and MMP-9. Our study showed for the first time that HCC-derived exosomes could mobilize normal hepatocyte, which may have implication in facilitating the protrusive activity of HCC cells through liver parenchyma during the process of metastasis.
Keyword	Hepatocellular carcinoma Liver cancer Carcinogenesis Metastasis Exosome Proteomics
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	16655
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Wong, N.
Recommended Citation GB/T 7714	He, M.,Qin, H.,Poon, T. C. W.,et al. Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs.[J]. Carcinogenesis., 2015, 1008-1018.
APA	He, M.., Qin, H.., Poon, T. C. W.., Sze, S. C.., Ding, X.., Co, N. N.., Ngai, S. M.., Chan, T. F.., & Wong, N. (2015). Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs.. Carcinogenesis., 1008-1018.
MLA	He, M.,et al."Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs.".Carcinogenesis. (2015):1008-1018.

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