Development of a Novel Method for Significant Improvement in Peptide De Novo Sequencing

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

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	Development of a Novel Method for Significant Improvement in Peptide De Novo Sequencing
	Ang, I. L.; Poon, C. W.
	2017-06-21
Source Publication	4th Macau Symposium on Biomedical Sciences 2017
Abstract	Background: In the field of proteomics, peptide de novo sequencing is the process of deriving amino acid sequences of peptides directly from tandem mass spectrometry data without the assistance of a protein sequence database. This strategy, however, is practically difficult and prone to have high error rate due to the presence of a complex mixture of fragment ions of various types (b-ions and y-ions) and uninterpretable ‘noise’ peaks in most tandem mass spectra. Method & Results: In this study, we developed a method to chemically modify tryptic peptides. The modified peptides were shown to provide clear and consecutive y-ions series in the tandem mass spectra. Moreover, a good discrimination of y-ions from b-ions was obtained. For the unmodified peptides, the accuracy of the de novo sequencing results was <60%. After modification, the accuracy was increased to >90%. Conclusion: A new peptide modification method for achieving high accuracy in peptide de novo sequencing was developed. To the best of our knowledge, y-ions and b-ions had not been successfully differentiated before this study. This method has great potentials in protein sequencing analysis when the relevant protein sequence database is not available.
Keyword	Proteomics de novo sequencing mass spectrometry
Language	英語English
The Source to Article	PB_Publication
PUB ID	33379
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Poon, C. W.
Recommended Citation GB/T 7714	Ang, I. L.,Poon, C. W.. Development of a Novel Method for Significant Improvement in Peptide De Novo Sequencing[C], 2017.
APA	Ang, I. L.., & Poon, C. W. (2017). Development of a Novel Method for Significant Improvement in Peptide De Novo Sequencing. 4th Macau Symposium on Biomedical Sciences 2017.

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