Regulatory effects of DCA, one component of anti inflammatory traditional Chinese medicine-Niuhuang, on human leukocyte response to chemoattractants

UM > Institute of Chinese Medical Sciences

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	Regulatory effects of DCA, one component of anti inflammatory traditional Chinese medicine-Niuhuang, on human leukocyte response to chemoattractants
	Chen, X.; Mellon, R.; Yang, L.; Dong, H.; Oppenheim, J.; Howard, O.
	2002-02-01
Source Publication	Biochemical Pharmacology
ISSN	0006-2952
Pages	533-541
Abstract	Niuhuang is a commonly used Chinese traditional medicine with immunoregulatory and anti-inflammatory properties. Deoxycholic acid (DCA) is a major active constituent of Niuhuang. The reaction of human leukocytes to chemoattractants is an important part of the host immune response and also plays a crucial role in the development of inflammation. We, therefore, investigated the in vitro effects of DCA on human monocyte and neutrophil responses to classic chemoattractants [fMet-Leu-Phe (fMLP), complement fraction 5a (C5a)], CC chemokine [monocyte chemoattractant protein-1 (MCP-1/CCL2)], and/or CXC chemokines [stromal cell-derived factor-1 (SDF-1α/CXCL12), interleukin-8 (IL-8/CXCL8)]. The results showed that DCA significantly inhibited fMLP-induced monocyte and neutrophil chemotaxis and calcium mobilization, and also blocked the binding of [3H]fMLP and anti-formyl peptide receptor (FPR) monoclonal antibodies (mAb) to the cells. The inhibitory effects of DCA on calcium mobilization and anti-FPR-mAb binding to the receptor could be abrogated by washing DCA out of the cell suspension, suggesting that DCA blocked fMLP receptors via a steric hindrance mechanism, not via receptor internalization. DCA had no significant inhibitory effects on MCP-1-, SDF-1α-, or C5a-induced monocyte function, or C5a- or IL-8-induced neutrophil function. Taken together, our experimental results suggest that blockade of fMLP receptors may contribute to the anti-inflammatory effects of traditional medicine containing DCA.
Keyword	Deoxycholic acid (DCA)Human leukocyteChemokine/chemoattractantChemotaxisCalcium mobilizationLigand–receptor binding
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	34893
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Howard, O.
Recommended Citation GB/T 7714	Chen, X.,Mellon, R.,Yang, L.,et al. Regulatory effects of DCA, one component of anti inflammatory traditional Chinese medicine-Niuhuang, on human leukocyte response to chemoattractants[J]. Biochemical Pharmacology, 2002, 533-541.
APA	Chen, X.., Mellon, R.., Yang, L.., Dong, H.., Oppenheim, J.., & Howard, O. (2002). Regulatory effects of DCA, one component of anti inflammatory traditional Chinese medicine-Niuhuang, on human leukocyte response to chemoattractants. Biochemical Pharmacology, 533-541.
MLA	Chen, X.,et al."Regulatory effects of DCA, one component of anti inflammatory traditional Chinese medicine-Niuhuang, on human leukocyte response to chemoattractants".Biochemical Pharmacology (2002):533-541.

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