Phenotypic changes in ifn-gamma 3'UTR are knock out mice

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Phenotypic changes in ifn-gamma 3'UTR are knock out mice
	Deborah , H.; Cyril, B.; Jeff, S.; Veronique, P.; William, B.; Xin, C.; Matthew, B.; Thomas, W.; Howard, Y.
	2007-08-01
Source Publication	JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
Abstract	Interferon (IFN) gamma is a pleotrophic cytokine that impacts a variety of immune functions. The importance of this cytokine requires tight control of its expression. This regulation is complex and involves both transcriptional and post-transcriptional mechanisms. The 3 untranslated region (3 UTR) of the IFNgamma mRNA contains a highly conserved 100 nucleotide sequence. Embedded in this sequence are multiple AU-rich elements (AREs) which function in mRNA stabilization and translational silencing. To study the impact of these sequences on IFN gamma expression, we have created an IFN-gamma ARE knock-out mouse. Elimination of the ARE does not impair transcription nor does it inhibit functional IFN gamma protein synthesis. Mice lacking the IFN-gamma-specific ARE have chronic low levels of IFN-gamma in their serum. Additionally, upon IL-12 stimulation, these mice produce 3-5-fold more IFNgamma than their wild type littermate controls. The mice have increased cellularity in both spleen and liver which is due to increased numbers of NKT, CD4 and CD8 T cells in both organs. In thymi, the percentage of CD4 and CD8 T cells are increased and these cells express lower levels of CD24 and CD69 suggesting a more mature T cell phenotype. Currently, a relationship between IFN-gamma production and immune development has not been established but studies are underway to investigate the impact of chronic IFN-gamma secretion on multiple levels of immunological development and function.
Keyword	Phenotypic changes ifn-gamma 3'UTR knock out
Language	英語English
The Source to Article	PB_Publication
PUB ID	47014
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Recommended Citation GB/T 7714	Deborah , H.,Cyril, B.,Jeff, S.,et al. Phenotypic changes in ifn-gamma 3'UTR are knock out mice[C], 2007.
APA	Deborah , H.., Cyril, B.., Jeff, S.., Veronique, P.., William, B.., Xin, C.., Matthew, B.., Thomas, W.., & Howard, Y. (2007). Phenotypic changes in ifn-gamma 3'UTR are knock out mice. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH.

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