Panax Notoginseng Protects against Diabetes-associated Endothelial Dysfunction: Comparison between Ethanolic Extract and Total Saponin

UM > Institute of Chinese Medical Sciences

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	Panax Notoginseng Protects against Diabetes-associated Endothelial Dysfunction: Comparison between Ethanolic Extract and Total Saponin
	Zhang, X.; Zhou, C.; Miao, L.; Tan, Y.; Zhou, Y.; Cheong, M. S. ; Huang, Y.; Wang, Y. T.; Yu, H.; Cheang, W. S.
	2021-09-06
Source Publication	Oxidative Medicine and Cellular Longevity
ISSN	1942-0994
Pages	4722797-4722797
Abstract	Previous studies revealed a cardioprotective potential of Panax notoginseng to relieve acute myocardial infarction and focal cerebral ischemia-reperfusion. However, whether P. notoginseng protects endothelial function in diabetes and the underlying mechanisms remain to be explored. P. notoginseng contains several chemical components including saponins, which are commonly believed as the major bioactive ingredients. The present study aimed to examine and compare the vaso-protective effects of the ethanolic extract of P. notoginseng (PNE) and total saponin (PNS). Both aortas and carotid arteries were isolated from male C57BL/6J mice for ex vivo treatment with risk factors (high glucose or tunicamycin) with and without presence of PNS and PNE. Diabetic model was established by feeding the mice with high-fat diet (45% kcal% fat) for 12 weeks while PNS and PNE were administrated by oral gavage at 20 mg/kg/day for another 4 weeks. Ex vivo exposure to high glucose impaired acetylcholine-induced endothelium-dependent relaxations in mouse aortas, decreased phosphorylation of AMPK and eNOS, and induced endoplasmic reticulum (ER) stress and oxidative stress. These effects were reversed by co-treatment of PNS and PNE with PNS being more potent. Furthermore, the vaso-protective effects were abolished by Compound C (AMPK inhibitor). Chronic treatment with PNS and PNE improved endothelium-dependent relaxations and alleviated ER stress and oxidative stress in aortas from high-fat diet-induced obese mice. PNE was more effective to improve glucose sensitivity and normalize blood pressure in diabetic mice. The present results showed that PNS and PNE reduced ER stress and oxidative stress, and subsequently improved endothelial function in diabetes through AMPK activation. This study provides new inspiration on the therapeutic potential of P. notoginseng extract against vascular diseases associated with metabolic disorders.
Keyword	panax notoginseng diabetes endothelial dysfunction endoplasmic reticulum stress saponins
Language	英語English
The Source to Article	PB_Publication
PUB ID	60545
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Cheang, W. S.
Recommended Citation GB/T 7714	Zhang, X.,Zhou, C.,Miao, L.,et al. Panax Notoginseng Protects against Diabetes-associated Endothelial Dysfunction: Comparison between Ethanolic Extract and Total Saponin[J]. Oxidative Medicine and Cellular Longevity, 2021, 4722797-4722797.
APA	Zhang, X.., Zhou, C.., Miao, L.., Tan, Y.., Zhou, Y.., Cheong, M. S. ., Huang, Y.., Wang, Y. T.., Yu, H.., & Cheang, W. S. (2021). Panax Notoginseng Protects against Diabetes-associated Endothelial Dysfunction: Comparison between Ethanolic Extract and Total Saponin. Oxidative Medicine and Cellular Longevity, 4722797-4722797.
MLA	Zhang, X.,et al."Panax Notoginseng Protects against Diabetes-associated Endothelial Dysfunction: Comparison between Ethanolic Extract and Total Saponin".Oxidative Medicine and Cellular Longevity (2021):4722797-4722797.

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