This study conceptualize craftsman spirit and proposes it as a contributor to individuals’ career and well-being. We define craftsman spirit as a work ethic that denotes striving to be the true self and pursuing perfection at work with the aims to achieve self-actualization from work and contribute to the well-being of others. Seven dimensions (i.e., authenticity, job dedication, positive perfectionism, creativity motive, harmonious work passion, resilience, and citizenship) are identified to elaborately describe the features of craftsman spirit. Specifically, the study proposes the positive influence of craftsman spirit on individuals’ career satisfaction, career progression, job performance, and subjective well-being. The implications and future research directions of this new construct are discussed.