Developing effective siRNAs to reduce the expression of key viral genes of COVID-19

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Developing effective siRNAs to reduce the expression of key viral genes of COVID-19
	Wu, R.; Luo, K.Q.
	2021-04-10
Source Publication	International Journal of Biological Sciences
ISSN	1449-2288
Pages	1521-1529
Abstract	The COVID-19 pandemic has been raging worldwide for more than a year. Many efforts have been made to create vaccines and develop new antiviral drugs to cope with the disease. Here, we propose the application of short interfering RNAs (siRNAs) to degrade the viral genome, thus reducing viral infection. By introducing the concept of the probability of binding efficiency (PBE) and combining the secondary structures of RNA molecules, we designed 11 siRNAs that target the consensus regions of three key viral genes: the spike (S), nucleocapsid (N) and membrane (M) genes of SARS-CoV-2. The silencing efficiencies of the siRNAs were determined in human lung and endothelial cells overexpressing these viral genes. The results suggested that most of the siRNAs could significantly reduce the expression of the viral genes with inhibition rates above 50% in 24 hours. This work not only provides a strategy for designing potentially effective siRNAs against target genes but also validates several potent siRNAs that can be used in the clinical development of preventative medication for COVID-19 in the future.
Keyword	COVID-19 SARS-CoV-2 RNA secondary structure siRNA gene silencing
Language	英語English
The Source to Article	PB_Publication
PUB ID	59841
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Luo, K.Q.
Recommended Citation GB/T 7714	Wu, R.,Luo, K.Q.. Developing effective siRNAs to reduce the expression of key viral genes of COVID-19[J]. International Journal of Biological Sciences, 2021, 1521-1529.
APA	Wu, R.., & Luo, K.Q. (2021). Developing effective siRNAs to reduce the expression of key viral genes of COVID-19. International Journal of Biological Sciences, 1521-1529.
MLA	Wu, R.,et al."Developing effective siRNAs to reduce the expression of key viral genes of COVID-19".International Journal of Biological Sciences (2021):1521-1529.

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