| Status | 已發表Published |
| Glycyrrhetinic acid induces a protective autophagy in HepG2 hepatocellular carcinoma cells by activating ERK pathway | |
Tang, ZH ; Li, T; Sun, W; Chang, LL; Zhu, H; Chen, X. P.; Wang, Y. T.; Lu, J. 
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| 2014-09-01 | |
| Source Publication | The 8th Chinese Conference on Oncology
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| Abstract | Background: Glycyrrhetinic acid (GA), isolated from the Chinese medicinal herb named Glycyrrhiza, is a triterpenoid saponin with well-known anti-cancer effects. In this study, we aimed to study the effects and mechanisms of GA-induced autophagy in HepG2 cells. Methods: The anti-proliferative effect of GA in HepG2 cells was examined by MTT assay. The GA-induced autophagy was determined by monodansylcadaverine (MDC) staining, transmission electron microscopy (TEM) observation, and western blot analysis of lipid modification of light chain-3 (LC3) expression. The autophagy-related signaling molecules and extracellular regulated protein kinases (ERK) were examined by western blot analysis. The role of autophagy in GA-induced cell proliferative inhibition and apoptosis of HepG2 cells was assessed by MTT and western blot. Results: GA concentration-dependently inhibited the proliferation of HepG2 cells. It obviously induced autophagosomes formation, up-regulates the LC3II expression, and activated ERK pathway in a concentration-dependent manner. Whereas, pretreatment of mitogen-activated protein kinase kinase 1/2 (MEK1/2) inhibitors U0126 or PD98059, the GA-induced autophagy was remarkably reversed as supported by the reduction of autophagic protein marker LC3II expression. Additionally, the anti-proliferative and apoptotic inducing potential of GA were enhanced by pretreatment of autophagic inhibitors chloroquine or bafilomycin A1. Conclusion: To our knowledge, this is the first time to demonstrate that GA induces protective autophagy by activating the ERK pathway in HepG2 cells which shed a new light for the future application of GA as a new anti-cancer compound. |
| Keyword | Glycyrrhetinic acid hepatocellular carcinoma autophagy ERK |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 34462 |
| Document Type | Conference paper |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | Lu, J. |
| Recommended Citation GB/T 7714 | Tang, ZH,Li, T,Sun, W,et al. Glycyrrhetinic acid induces a protective autophagy in HepG2 hepatocellular carcinoma cells by activating ERK pathway[C], 2014. |
| APA | Tang, ZH., Li, T., Sun, W., Chang, LL., Zhu, H., Chen, X. P.., Wang, Y. T.., & Lu, J. (2014). Glycyrrhetinic acid induces a protective autophagy in HepG2 hepatocellular carcinoma cells by activating ERK pathway. The 8th Chinese Conference on Oncology. |
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