Baicalein triggers autophagy and activates the ERK pathway in ovarian carcinoma HEY cells

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Baicalein triggers autophagy and activates the ERK pathway in ovarian carcinoma HEY cells
	Wang, YF; Tang, ZH; Li, T; Zhang, LL; Chen, X. P.; Wang, Y. T.; Lu, J.
	2015-04-01
Source Publication	Chinese Society for Cell Biology-2015 National Congress and Academic Conference
Abstract	Baicalein (BA), one of the major compounds isolated from the root of Scutellaria baicalensis Gerogi, is a bioactive flavonoid with various pharmacological effects. In this study, we evaluated the autophagy triggered by BA on human ovarian carcinoma HEY cells. We found that BA inhibited the proliferation on HEY cells after 24 h treatment as evidenced by MTT assay. Meanwhile, BA triggered the acidic vesicular organelle manifested by monodansylcadaverine staining and enhanced the expression of LC3-II in HEY cells. Inhibition of autophagy by chloroquine (CQ), an inhibitor of autophagy, the cell viability was obviously decreased whereas the expression of cleaved PARP was significantly increased, which means that BA triggered a protective autophagy. We also found an increase in phosphorylation of p38, ERK and AKT after BA treatment. Furthermore, pretreatment with U0126, an MEK inhibitor, obviously inhibited BA-induced activation of ERK as well as that of LC3-II. These results suggest that BA triggers a protective autophagy via activation of ERK, which might attenuate its anticancer effects in ovarian carcinoma cells.
Keyword	baicalein ovarian carcinoma autophagy protective
Language	英語English
The Source to Article	PB_Publication
PUB ID	34460
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lu, J.
Recommended Citation GB/T 7714	Wang, YF,Tang, ZH,Li, T,et al. Baicalein triggers autophagy and activates the ERK pathway in ovarian carcinoma HEY cells[C], 2015.
APA	Wang, YF., Tang, ZH., Li, T., Zhang, LL., Chen, X. P.., Wang, Y. T.., & Lu, J. (2015). Baicalein triggers autophagy and activates the ERK pathway in ovarian carcinoma HEY cells. Chinese Society for Cell Biology-2015 National Congress and Academic Conference.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Affiliation No.
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh