Codelivery of Doxorubicin and shAkt1 by Poly ethylenimine)−Glycyrrhetinic Acid Nanoparticles To Induce Autophagy-Mediated Liver Cancer Combination Therapy

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	Codelivery of Doxorubicin and shAkt1 by Poly ethylenimine)−Glycyrrhetinic Acid Nanoparticles To Induce Autophagy-Mediated Liver Cancer Combination Therapy
	Wang, FZ; Xing, L; Tang, ZH; Lu, J.; Cui, PF; Qiao, JB; Jiang, L; Jiang, HL; Zhong, L
	2016-02-19
Source Publication	Molecular Pharmaceutics
ISSN	1543-8384
Pages	1298-1307
Abstract	Combination therapy has been developed as a promising therapeutic approach for hepatocellular carcinoma therapy. Here we report a low toxicity and high performance nanoparticle system that was self-assembled from a poly-(ethylenimine)−glycyrrhetinic acid (PEI−GA) amphiphilic copolymer as a versatile gene/drug dual delivery nanoplatform. PEI−GA was synthesized by chemical conjugation of hydrophobic GA moieties to the hydrophilic PEI backbone via an acylation reaction. The PEI−GA nanocarrier could encapsulate doxorubicin (DOX) efficiently with loading level about 12% and further condense DNA to form PEI−GA/DOX/DNA complexes to codeliver drug and gene. The diameter of the complexes is 102 ± 19 nm with zeta potential of 19.6 ± 0.2 mV. Furthermore, the complexes possess liver cancer targeting ability and could promote liver cancer HepG2 cell internalization. Apoptosis of cells could be induced by chemotherapy of DOX, and PI3K/Akt/mTOR signaling pathway acts a beneficial effect on the modulation of autophagy. Here, it is revealed that utilizing PEI−GA/DOX/shAkt1 complexes results in effective autophagy and apoptosis, which are useful to cause cell death. The induction of superfluous autophagy is reported to induce type-II cell death and also could increase the sensity of chemotherapy..
Keyword	combination therapy self-assembled glycyrrhetinic acid liver cancer targeting autophagy
Language	英語English
The Source to Article	PB_Publication
PUB ID	21037
Document Type	Journal article
Collection	University of Macau
Corresponding Author	Jiang, HL; Zhong, L
Recommended Citation GB/T 7714	Wang, FZ,Xing, L,Tang, ZH,et al. Codelivery of Doxorubicin and shAkt1 by Poly ethylenimine)−Glycyrrhetinic Acid Nanoparticles To Induce Autophagy-Mediated Liver Cancer Combination Therapy[J]. Molecular Pharmaceutics, 2016, 1298-1307.
APA	Wang, FZ., Xing, L., Tang, ZH., Lu, J.., Cui, PF., Qiao, JB., Jiang, L., Jiang, HL., & Zhong, L (2016). Codelivery of Doxorubicin and shAkt1 by Poly ethylenimine)−Glycyrrhetinic Acid Nanoparticles To Induce Autophagy-Mediated Liver Cancer Combination Therapy. Molecular Pharmaceutics, 1298-1307.
MLA	Wang, FZ,et al."Codelivery of Doxorubicin and shAkt1 by Poly ethylenimine)−Glycyrrhetinic Acid Nanoparticles To Induce Autophagy-Mediated Liver Cancer Combination Therapy".Molecular Pharmaceutics (2016):1298-1307.

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