| Status | 已發表Published |
| Vitamin C Induced Ca2+-Dependent Necrosis Mediated by ROS in Human Colorectal Cancer Cells | |
Wang, C.X. ; Yu, J.; Chen, J.Q.; Hou, Y.; Lu, J. ; Chen, X. P.
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| 2018-12-01 | |
| Source Publication | The 7th Guangzhou International Symposium on Oncology
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| Abstract | Vitamin C (VC) also known as ascorbic acid is a small molecular widely distributed in food and traditional Chinese medicines (TCM). It is an essential nutrient for human and is widely used as a dietary supplement. Recently, the anticancer effect of high concentration VC was documented. Here, we reported that high concentration VC induced cell death in HCT116 and HT29 colorectal cancer cells in concentration- and time- dependent manners. VC treatment induced ATP decrease, LDH release, cell swollen and loss of plasma membrane integrity. Pharmacological inhibitors for apoptosis, necroptosis, autophagy, pyroptosis and oncosis showed no effect on VC-induced cell death. Furthermore, increase of intracellular ROS and accumulation of calcium (Ca2+) were observed after VC treatment. N-Acetyl Cysteine (NAC) could attenuate both ROS increase and intracellular Ca2+ accumulation induced by VC, while BAPTA-AM could only attenuate intracellular Ca2+ accumulation. NAC, BAPTA-AM and 2-Aminoethoxydiphenyl borate (2-APB) could attenuate the cell death induced by VC. Interestingly, ferric iron (Fe3+) donor ferric citrate (FCA), ferric chloride (FeCl3), and ferrous iron donor ferrous sulfate (FeSO4) could dramatically inhibit VC-induced cell death. In addition, high concentration VC induced mitochondrial damage and mitochondrial ROS generation. Taken together, our results demonstrated that VC induced Ca2+-dependent programed necrosis mediated by ROS. Our study provided new insights into high concentration VC induced cell death in human colorectal cancer cells. |
| Keyword | Vitamin C ROS Ca2+ Fe3+/Fe2+ programed necrosis |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 46753 |
| Document Type | Conference paper |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | Chen, X. P. |
| Recommended Citation GB/T 7714 | Wang, C.X.,Yu, J.,Chen, J.Q.,et al. Vitamin C Induced Ca2+-Dependent Necrosis Mediated by ROS in Human Colorectal Cancer Cells[C], 2018. |
| APA | Wang, C.X.., Yu, J.., Chen, J.Q.., Hou, Y.., Lu, J.., & Chen, X. P. (2018). Vitamin C Induced Ca2+-Dependent Necrosis Mediated by ROS in Human Colorectal Cancer Cells. The 7th Guangzhou International Symposium on Oncology. |
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