Candida glabrata Xbp1 represses early transcription response during interaction with host macrophages

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Candida glabrata Xbp1 represses early transcription response during interaction with host macrophages
	Rai, N.; Parsania, C.; Rai, R.; Wong, K. H.
	2017-09-12
Source Publication	Microbial Pathogenesis & Host Response
Abstract	Candida glabrata, an opportunistic human fungal pathogen in immunocompromised patients, remodels its carbon metabolism to survive in nutrient-deprived intracellular macrophage microenvironment. Here, we have identified a transcription factor, CgXbp1, required for remodeling carbon metabolic pathways in C. glabrata upon exposure to host macrophages. Employing the next generation sequencing approaches, we showed that Cgxbp1Δ mutant exhibited constitutively active fatty acid catabolic and proteolytic pathways even in the presence of sufficient carbohydrate in the growth medium, suggesting that carbon metabolic processes are deregulated upon CgXBP1 deletion. Next, we demonstrated that CgXbp1 is essential for arresting the cells in G1 phase of cell cycle during carbon starvation. Global gene expression analysis on Cgxbp1Δ mutant revealed that genes associated with GO terms – mitochondrion organization, mitochondrial transport and mitochondrial translation were highly enriched among down-regulated genes, indicating that CgXbp1 may have pivotal roles in cellular energy homeostasis maintenance. Comparison of the transcriptional profiles of macrophage-engulfed wild-type and Cgxbp1Δ mutant cells revealed that unlike wild-type cells, Cgxbp1Δ mutant was unable to repress genes associated with GO terms - Translation, Ribosome biogenesis and RNA metabolic processes during early stage of macrophage infection. Finally, we performed time-course gene expression analyses in C. glabrata cells after phagocytosis by THP-1 macrophages, and demonstrated that Cgxbp1Δ mutant affects the temporal transcriptional response to macrophages. Overall, our data established that CgXbp1 is central in carbon metabolic remodeling during host-pathogen interactions in C. glabrata.
Keyword	Candida glabrata Xbp1 transcription host
Language	英語English
The Source to Article	PB_Publication
PUB ID	39963
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Rai, N.,Parsania, C.,Rai, R.,et al. Candida glabrata Xbp1 represses early transcription response during interaction with host macrophages[C], 2017.
APA	Rai, N.., Parsania, C.., Rai, R.., & Wong, K. H. (2017). Candida glabrata Xbp1 represses early transcription response during interaction with host macrophages. Microbial Pathogenesis & Host Response.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Affiliation No.
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh