Objective Hormonal abnormality may play a role in the development of overweight and obesity. The purpose of this study was to compare high-intensity interval exercise and moderate continuous exercise training on circulating GH and FGF-21 in overweight young women. Methodology 48 overweight young women including repeated-sprint exercise training (RSE, n=13), high-intensity interval exercise training (HIT, n=13), moderate continuous exercise training (MCT, n=14) and no exercise control (CON, n=8) completed a 12-week exercise intervention with four days each week. RSE comprised 6s of sprinting and 9s of rest with a maximum of 80 repetitions in 20 min. HIT performed 44min cycling at 85–95% HRpeak with 10-min recovery while MIT experienced moderate continuous cycling at 60–70% HRpeak with equivalent power output to HIT. Blood samples at a fasting state were assessed at pre- and post-intervention. Results & Discussion After exercise intervention, FGF-21 enhanced significantly (p=0.000). Subjects in HIT had a higher levels of FGF-21 than those of SIT, MCT and CON, whereas GH did not show any group differences. Leptin concentrations of post-measure in HIT and MCT were significantly lower than those in SIT and CON (p < 0.05). The increments of V̇O2peak were +24%, +27% and +26% in SIT,