Rapid Regulation of Oxidative Phosphorylation by Insulin in Human Embryonic Stem Cells.

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	Rapid Regulation of Oxidative Phosphorylation by Insulin in Human Embryonic Stem Cells.
	Ren, Z.; Zhong, H.; Song, C.; Deng, C.; Liu, W.; Chen, G.
	2019-12-07
Source Publication	ASCB/EMBO 2019 Meeting Abstracts: Poster Presentations
Abstract	Insulin is an extrinsic factor essential for human embryonic stem cell (hESC) maintenance and differentiation, and the functions are always carried out through transcription and translation over hours or even days. Emerging studies suggest that insulin is important for energy metabolism, which is increasingly recognized as an active control of cell functions, but in hESCs how insulin signal transduction influences energy balance and whether there is immediate impact are largely unknown. Here we show that the presence of insulin rapidly stimulates substrate‐specific oxidative phosphorylation (OXPHOS) in hESCs. Pharmacologic and genetic manipulations reveal that insulin regulates OXPHOS through PI3K/AKT/GSK3 cascade within minutes before significant changes in the expression of metabolic enzymes occur. Intriguingly, we observed that insulin‐dependent OXPHOS is hESC‐specific, and it diminishes in differentiated cell types. Moreover, we noticed that insulin or GSK3 inhibition could significantly suppress the activation of caspase. Collectively, these results suggest that insulin regulated OXPHOS in a rapid mode and insulin is continuously required to maintain full mitochondrial capacity in hESCs. Our findings provide molecular insights into the association of insulin signal transduction and mitochondrial energy metabolism, and highlight the necessity of insulin in the consistency and accuracy of metabolic analyses in intact hESCs.
Keyword	hESC Insulin Oxidative phosphorylation PI3K/AKT/GSK3
Language	英語English
The Source to Article	PB_Publication
PUB ID	49625
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Chen, G.
Recommended Citation GB/T 7714	Ren, Z.,Zhong, H.,Song, C.,et al. Rapid Regulation of Oxidative Phosphorylation by Insulin in Human Embryonic Stem Cells.[C], 2019.
APA	Ren, Z.., Zhong, H.., Song, C.., Deng, C.., Liu, W.., & Chen, G. (2019). Rapid Regulation of Oxidative Phosphorylation by Insulin in Human Embryonic Stem Cells.. ASCB/EMBO 2019 Meeting Abstracts: Poster Presentations.

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