| Status | 已發表Published |
| EIF4A1-MEDIATED SPECIFIC PROTEIN TRANSLATION IN HUMAN EMBRYONIC STEM CELL | |
Zhou, X. ; Ren, Z.; Liu, W. ; Chen, G.
| |
| 2019-06-26 | |
| Source Publication | ISSCR 2019 Annual Meeting
 |
| Abstract | Protein translation is critical for numerous cellular functions such as transcription, metabolism, stress response and cellular proliferation. However, it is unclear how translation is regulated in human embryonic stem cells(hESC), and how it affects specific cellular functions. In this report, we show that eIF4A1-dependent translation initiation plays critical roles in hESC maintenance. EIF4A1 is essential for Cap-dependent translation, and its inhibition by small chemical silverstrol leads to severe cell type specific cell death in hESC, but not in other somatic cells. Genome-wide ribosome profiling analysis reveals that silvestrol treatment specifically suppresses pathways involved in TGFβ signaling, MAPK signaling, apoptosis and so on. Further study shows that silvestrol specifically suppressed NANOG and CDH1; but not OCT4 and SOX2. These data show eIF4A1-mediated translation differentially control pluripotency related proteins, and influence stem cell survival and cell fate determination. |
| Keyword | Translation hESC pluripotency |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 47190 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES |
| Corresponding Author | Chen, G. |
| Recommended Citation GB/T 7714 | Zhou, X.,Ren, Z.,Liu, W.,et al. EIF4A1-MEDIATED SPECIFIC PROTEIN TRANSLATION IN HUMAN EMBRYONIC STEM CELL[C], 2019. |
| APA | Zhou, X.., Ren, Z.., Liu, W.., & Chen, G. (2019). EIF4A1-MEDIATED SPECIFIC PROTEIN TRANSLATION IN HUMAN EMBRYONIC STEM CELL. ISSCR 2019 Annual Meeting. |
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