Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes
	Li, Z.; Zhang, P.; Zhang, R.; Zhang, H.
	2019-06-01
Source Publication	22nd International C. elegans Conference Abstract Book
Abstract	Localized mRNAs contribute to targeting proteins to discrete sites and are required for diverse cellular and developmental events, including translation, cell division, cell motility and cell-fate determination. The ability to visualize mRNA in real time at high resolution in live animals are critical for full understanding the spatiotemporal dynamics of gene regulation and function. Here we report a method to visualize mRNA using PP7-PCP system in C. elegans, in which mRNAs containing bacteriophage PP7 binding sites are labeled by the PP7 coat protein fused to fluorescent reporters. Using this approach, we construct a collection of tissue-specific, differentially expressed, membrane-associated mRNA toolkit strains and detect distinct mRNA dynamics and localizations through monitoring single RNP particles. We have applied this system to dissect the cis-regulatory element for mRNA localization and explore development- and tissue-dependent mRNA-protein localization correlations. Furthermore, we attempt to use the PP7-PCP RNA labeling system to uncover the possible link between mRNA localization and epithelial polarity in C. elegans. Together our results outline a powerful methodology to monitor mRNA through its life cycle. This approach offers important advantages compared with other gene expression techniques already available in this experimentally tractable model organism.
Keyword	C. elegans transcripts membrane-associated genes
Language	英語English
The Source to Article	PB_Publication
PUB ID	46797
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Recommended Citation GB/T 7714	Li, Z.,Zhang, P.,Zhang, R.,et al. Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes[C], 2019.
APA	Li, Z.., Zhang, P.., Zhang, R.., & Zhang, H. (2019). Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes. 22nd International C. elegans Conference Abstract Book.

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