Sphingolipidomic Analysis of C. elegans Reveals Development- and Growth Condition-Dependent Metabolic Features

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

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	Sphingolipidomic Analysis of C. elegans Reveals Development- and Growth Condition-Dependent Metabolic Features
	Cheng, X.; Jiang, X.; Zhang, H.
	2019-06-01
Source Publication	22nd International C. elegans Conference Abstract Book
Abstract	Sphingolipids (SLs) serve as structural and signaling molecules in regulating various cellular events and growth. Given that SLs contain various bioactive species possessing distinct roles, quantitative analysis of sphingolipidome is essential for elucidating their differential requirement during development. Herein we developed a comprehensive Sphingolipidomic profiling approach using liquid chromatography (LC) - mass spectrometry (MS) coupled with multiple reaction monitoring (MRM) analysis. SL profiling of C. elegans using this approach revealed organism-specific, development-dependent and environment-driven metabolic features. We showed for the first time a predominance of ceramide-1-phosphate (C1P) in the C. elegans sphingolipidome, and the presence of a series of sphingoid bases and 1-deoxy-sphingoid bases. Moreover, we were able to resolve growth-, temperature- and nutrition-dependent SL profiles at both individual metabolite-level and network-level. Sphingolipidomic analysis revealed significant SL composition changes throughout development, with GluCers/C1Ps ratios increasing from L1- to L2-stage followed by a gradual decrease thereafter. We also identified a temperature-dependent alteration in C1Ps/(GluCers+SMs), suggesting an organism-specific strategy for environmental adaptation. Finally, we found a significant serine-biased GluCer increase between serine- versus alanine-supplemented worms. This analysis provides a baseline characterization of the biochemical network and establishes LC-MS-MRM based sphingolipidomics as a powerful platform for complementing genetics studies in worms.
Keyword	Sphingolipidomics C. elegans Metabolic Features
Language	英語English
The Source to Article	PB_Publication
PUB ID	46795
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Recommended Citation GB/T 7714	Cheng, X.,Jiang, X.,Zhang, H.. Sphingolipidomic Analysis of C. elegans Reveals Development- and Growth Condition-Dependent Metabolic Features[C], 2019.
APA	Cheng, X.., Jiang, X.., & Zhang, H. (2019). Sphingolipidomic Analysis of C. elegans Reveals Development- and Growth Condition-Dependent Metabolic Features. 22nd International C. elegans Conference Abstract Book.

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