Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

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	Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis
	Zhang, H.; Kim, A.; Abraham, N.; Hall, D.; Fleming, J.; Gobel, V.
	2012-04-25
Source Publication	Development
ISSN	0951-1991
Pages	2071-2083
Abstract	Clathrin coats vesicles in all eukaryotic cells and has a well-defined role in endocytosis, moving molecules away from the plasma membrane. Its function on routes towards the plasma membrane was only recently appreciated and is thought to be limited to basolateral transport. Here, an unbiased RNAi-based tubulogenesis screen identifies a role of clathrin (CHC-1) and its AP-1 adaptor in apical polarity during de novo lumenal membrane biogenesis in the C. elegans intestine. We show that CHC-1/AP-1-mediated polarized transport intersects with a sphingolipid-dependent apical sorting process. Depleting each presumed trafficking component mislocalizes the same set of apical membrane molecules basolaterally, including the polarity regulator PAR-6, and generates ectopic lateral lumens. GFP::CHC-1 and BODIPY-ceramide vesicles associate perinuclearly and assemble asymmetrically at polarized plasma membrane domains in a co-dependent and AP-1-dependent manner. Based on these findings, we propose a trafficking pathway for apical membrane polarity and lumen morphogenesis that implies: (1) a clathrin/AP-1 function on an apically directed transport route; and (2) the convergence of this route with a sphingolipid-dependent apical trafficking path.
Keyword	Caenorhabditis elegans Polarity Tubulogenesis Clathrin AP-1 Sphingolipids
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	53814
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Gobel, V.
Recommended Citation GB/T 7714	Zhang, H.,Kim, A.,Abraham, N.,et al. Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis[J]. Development, 2012, 2071-2083.
APA	Zhang, H.., Kim, A.., Abraham, N.., Hall, D.., Fleming, J.., & Gobel, V. (2012). Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis. Development, 2071-2083.
MLA	Zhang, H.,et al."Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis".Development (2012):2071-2083.

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