| Status | 已發表Published |
| Mouse Interleukin-2 Receptor Graphic Gene Expression | |
Wang, S. M.
| |
| 1995-05-05 | |
| Source Publication | Journal of Biological Chemistry
 |
| ISSN | 1083-351X |
| Pages | 10743-10753 |
| Abstract | We have shown that interleukin-1 (IL-1) and IL-2 control IL-2 receptor α (IL-2Rα) gene transcription in CD4GraphicCD8Graphic murine T lymphocyte precursors. Here we map the cis-acting elements that mediate interleukin responsiveness of the mouse IL-2Rα gene using a thymic lymphoma-derived hybridoma (PC60). The transcriptional response of the IL-2Rα gene to stimulation by IL-1 + IL-2 is biphasic. IL-1 induces a rapid, protein synthesis-independent appearance of IL-2Rα mRNA that is blocked by inhibitors of NF-κB activation. It also primes cells to become IL-2 responsive and thereby prepares the second phase, in which IL-2 induces a 100-fold further increase in IL-2Rα transcripts. Transient transfection experiments show that several elements in the promoter-proximal region of the IL-2Rα gene contribute to IL-1 responsiveness, most importantly an NF-κB site conserved in the human and mouse gene. IL-2 responsiveness, on the other hand, depends on a 78-nucleotide segment 1.3 kilobases upstream of the major transcription start site. This segment functions as an IL-2-inducible enhancer and lies within a region that becomes DNase I hypersensitive in normal T cells in which IL-2Rα expression has been induced. IL-2 responsiveness requires three distinct elements within the enhancer. Two of these are potential binding sites for STAT proteins. |
| Keyword | T cell interleukin 2 receptor regulation |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 43509 |
| Document Type | Journal article |
| Collection | DEPARTMENT OF PUBLIC HEALTH AND MEDICINAL ADMINISTRATION |
| Recommended Citation GB/T 7714 | Wang, S. M.. Mouse Interleukin-2 Receptor Graphic Gene Expression[J]. Journal of Biological Chemistry, 1995, 10743-10753. |
| APA | Wang, S. M..(1995). Mouse Interleukin-2 Receptor Graphic Gene Expression. Journal of Biological Chemistry, 10743-10753. |
| MLA | Wang, S. M.."Mouse Interleukin-2 Receptor Graphic Gene Expression".Journal of Biological Chemistry (1995):10743-10753. |
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