HSQ14 inhibits NLRP3 inflammasome activation by preserving mitochondrial integrity and augmenting SIRT3-mediated autophagy

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	HSQ14 inhibits NLRP3 inflammasome activation by preserving mitochondrial integrity and augmenting SIRT3-mediated autophagy
	Zhang, T.; Fang, Z.; Wang, Y. T.; Gan, L.; Lin, L.
	2019-08-08
Source Publication	Abstract of 18th Meeting of Consortium for Globalization of Chinese Medicine
Abstract	The nod-like receptor 3 (NLRP3) inflammasome is a caspase-1-containing multi-protein complex that controls the release of interleukin-1β (IL-1β) and plays important roles in the development of inflammatory diseases. Our previous study reported that HSQ14, a cycloartane triterpenoid isolated from the whole plant of Actaea vaginata (Ranunculaceae), possesses anti-inflammatory effect on lipopolysaccharide (LPS)-treated RAW264.7 macrophages. However, the underlying mechanism hasn’t been clarified clearly. Herein, we investigated the effect of HSQ14 on mitochondrial integrity and NLRP3 inflammasome activation in vitro and in vivo. HSQ14 alleviated LPS-induced inflammation in RAW264.7 cells through activating SIRT3-mediated autophagy. Furthermore, HSQ14 inhibited the activation of NLRP3 inflammasome by enhancing mitophagy and ameliorating mitochondrial damage. In addition, upregulation of AMPK/ULK1 signaling pathway accounted for HSQ14-driven autophagy. HSQ14 treatment alleviated LPS-induced acute inflammation in C57BL/6 mice, indicating by the inactivation of NLRP3 inflammasome and suppression of pro-inflammatory cytokines, including IL-1β, tumor necrosis factor-α, IL-6 and monocyte chemoattractant protein-1. In conclusion, our results demonstrated that HSQ14 might be potentially applied for the treatment of NLRP3-associated inflammatory diseases.
Keyword	SIRT3 adipocytes autophagy chaperon mediated autophagy AMPK
Language	英語English
The Source to Article	PB_Publication
PUB ID	49993
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lin, L.
Recommended Citation GB/T 7714	Zhang, T.,Fang, Z.,Wang, Y. T.,et al. HSQ14 inhibits NLRP3 inflammasome activation by preserving mitochondrial integrity and augmenting SIRT3-mediated autophagy[C], 2019.
APA	Zhang, T.., Fang, Z.., Wang, Y. T.., Gan, L.., & Lin, L. (2019). HSQ14 inhibits NLRP3 inflammasome activation by preserving mitochondrial integrity and augmenting SIRT3-mediated autophagy. Abstract of 18th Meeting of Consortium for Globalization of Chinese Medicine.

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