Coordinated  regulation  of  gluconeogenesis,  glyoxylate  bypass  and  TCA  cycle  genes  through  promoter  specific  cooperation  among  AcuK,  AcuM  and  FacB  in Aspergillus  nidulans.

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	Coordinated regulation of gluconeogenesis, glyoxylate bypass and TCA cycle genes through promoter specific cooperation among AcuK, AcuM and FacB in Aspergillus nidulans.
	Dong, L.; Wong, K. H.
	2018-06-08
Source Publication	Macau Symposium on Biomedical Sciences
Abstract	Date: 8th-9th June, 2018 Venue: University of Macau Enquiry: [email protected] Macau Symposium on Biomedical Sciences 2018 Faculty of Health Sciences, University of Macau Contact information of first author or presenter: Name: Dong Liguo Salute (Mr.):Affiliation: Faculty of Health Science, University of Macau Advisor’s name or correspondence: Chris, Koon Ho Wong Contact telephone: +853 66727447 Contact email: [email protected] title (Max 250 characters): Coordinated regulation of gluconeogenesis, glyoxylate bypass and TCA cycle genes through promoter specific cooperation among AcuK, AcuM and FacB in Aspergillus nidulansAbstract (Max 250 words): Carbon metabolism plays central roles for fungal survival. From the preferred carbon sources (like glucose) to the non-favorable carbon sources (like acetate), fungi are required to alter metabolism processes for utilization of different carbon sources through precisely controlling expression of specific genes. Genetic studies in Aspergillus nidulans show that the transcription factors AcuK, AcuM and FacB regulate genes required for acetate metabolism. Specifically, the AcuK and AcuM heterodimer regulates expression of enzymes for gluconeogenesis, while FacB controls glyoxylate bypass genes. However, the full spectrum of targeted genes and transcription regulatory mechanisms of AcuK, AcuM and FacB were not fully understood because only limited numbers of genes were studied previously. In this study, we aimed to dissect the genome-wide roles and transcription regulation mechanisms of the three above-mentioned transcription factors using genomic approaches such as ChIP-seqs and RNA-seqs. Our result shows that AcuK/AcuM and FacB cooperatively regulate a large set of overlapping genes involved in gluconeogenesis and glyoxylate bypass pathways. Interestingly, we found that both AcuK-AcuM and FacB could alter histone density to facilitate binding of each other, but they do so in a promoter specific manner with FacB helping AcuK-AcuM binding at glyoxylate bypass genes while vice versa at gluconeogenesis genes. On the other hand, similar cooperation relationships between AcuK/AcuM and FacB are not observed at the promoter of TCA cycle genes. We suspect that other transcription factors may be involved in regulating TCA cycle genes. Our preliminary results show that E-box and 5’-CCAAT-3’ motifs are enriched on or around AcuK/AcuM and FacB summits, suggesting that HLH family transcription factors (binding on E-box) and AnCF complexes (binding on CCAAT motifs) are probable candidates. In order to fully understand AcuK/AcuM and FacB functions in regulating TCA cycle, we will study the involvement of HLH transcription factors and AnCF complexes with AcuK/AcuM and FacB regulation. Overall, this project will figure out how AcuK, AcuM, FacB and other regulators cooperatively control carbon metabolism in Aspergillus nidulans.
Keyword	regulation gluconeogenesis glyoxylate bypass TCA cycle genes promoter Aspergillus nidulans
Language	英語English
The Source to Article	PB_Publication
PUB ID	40071
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Dong, L.,Wong, K. H.. Coordinated regulation of gluconeogenesis, glyoxylate bypass and TCA cycle genes through promoter specific cooperation among AcuK, AcuM and FacB in Aspergillus nidulans.[C], 2018.
APA	Dong, L.., & Wong, K. H. (2018). Coordinated regulation of gluconeogenesis, glyoxylate bypass and TCA cycle genes through promoter specific cooperation among AcuK, AcuM and FacB in Aspergillus nidulans.. Macau Symposium on Biomedical Sciences.

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