Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative injured L02 cells

UM > Institute of Chinese Medical Sciences

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	Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative injured L02 cells
	Wang, A. Q.; Wang, S. P.; Jiang, Y.; Chen, M. W.; Wang, Y. T.; Lin, L. G.
	2016-08-20
Source Publication	15th Meeting of Consortium for Globalization of Chinese Medicine (CGCM)
Abstract	Penthorum chinense Pursh (Ganhuangcao in Chinese) is traditionally used for liver protection and treatment of liver diseases, including hepatitis B, hepatitis C and alcoholic liver damage. We and others have disclosed the hepatoprotective effect of different extracts from P. chinense. To further explore the chemical principles responsible for its liver protective effect, a bioactivity guided isolation was carried out on the water extract of P. chinense, which lead to identification of an effective fraction (E50M60). Further isolation of E50M60 fraction obtained ten polyphenols. Among them, quercetin showed the most significant protective effect on tert-butyl hydroperoxide (t-BHP) induced hepatocytes damage. mechanism study demonstrated that both the E50M60 fraction and quercetin attenuated t-BHP induced hepatocytes apoptosis through up-regulating the expression of B-cell lymphoma-2 protein (BCL-2) and BCL-xL, and down-regulating the cleaved products of caspase-7, -9 and nuclear poly (ADP-ribose) polymerase (PARP). Moreover, the E50M60 fraction and quercetin promoted nuclear factor-like 2 (NRF2), superoxide dismutase-2 (SOD-2) and heme oxygenase-1 (HO-1) expressions and suppressed Kelch-like ECH-associated protein 1 (KEAP-1) expression, resulting in resistance to the reactive oxygen species (ROS) induced mitochondrial oxidative stress. Taken together, both the active fraction and quercetin from P. chinense might be well developed as novel functional food for liver protection.
Keyword	Penthorum chinense hepatoprotective ROS NRF2
Language	英語English
The Source to Article	PB_Publication
PUB ID	29014
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lin, L. G.
Recommended Citation GB/T 7714	Wang, A. Q.,Wang, S. P.,Jiang, Y.,et al. Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative injured L02 cells[C], 2016.
APA	Wang, A. Q.., Wang, S. P.., Jiang, Y.., Chen, M. W.., Wang, Y. T.., & Lin, L. G. (2016). Bio-assay guided identification of hepatoprotective polyphenols from Penthorum chinense Pursh on t-BHP induced oxidative injured L02 cells. 15th Meeting of Consortium for Globalization of Chinese Medicine (CGCM).

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