Comprehensive analysis of chromatin landscape in pathogenic and non-pathogenic fungi.

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Comprehensive analysis of chromatin landscape in pathogenic and non-pathogenic fungi.
	Wang, X.; Parsania, C.; Wang, F.; Sethiya, P.; Qin, L.; Rai, N.; Djordjevic, D.; Tan, K.; Ho, J.; Wong, K. H.
	2018-06-11
Source Publication	DNA Metabolism, Genomic Stability & Human Disease
Abstract	Eukaryotic genome consists of chromatin, which composes of DNA and a large set of DNA-interacting histone and nonhistone proteins. Chromatin organisation, such as the deposition of active or repressive histone modifications, plays an important role in regulating gene expression. Advances in Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) and associated bioinformatics analytical techniques have enabled genome-wide analysis of chromatin modification and transcription regulation dynamics. The chromatin landscape of human, mouse, and several model organisms have been widely studied, but other medically and biotechnologically important species, including many fungal species, are not yet fully studied. Using ChIP-seq, we generated genome-wide profiles of H3, H3K4me3, H3K4me1, H3K27ac, H3K9me3, H3K9ac, PolII and transcription factors in a number of different fungal species under different growth conditions as a novel approach to dissect the regulation of cellular metabolic homeostasis. All sequences were mapped to the reference genome, and processed using the standard ChIP-seq analysis pipeline SPP. We used a recently developed hierarchically linked infinite hidden Markov model (hiHMM [1]) to systematically discover and characterise the most prevalent combination of histone modifications, i.e., chromatin state. Our work provides a valuable resource for a number of fungal species that opens new avenues for investigation of the dynamic chromatin organisation and gene regulation in fungi.
Keyword	Histones CHIP-seq transcription factors fungi pathogenic
Language	英語English
The Source to Article	PB_Publication
PUB ID	39951
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Wang, X.,Parsania, C.,Wang, F.,et al. Comprehensive analysis of chromatin landscape in pathogenic and non-pathogenic fungi.[C], 2018.
APA	Wang, X.., Parsania, C.., Wang, F.., Sethiya, P.., Qin, L.., Rai, N.., Djordjevic, D.., Tan, K.., Ho, J.., & Wong, K. H. (2018). Comprehensive analysis of chromatin landscape in pathogenic and non-pathogenic fungi.. DNA Metabolism, Genomic Stability & Human Disease.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Affiliation No.
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh