Bioassay coupled fractionation for development of anti-HCV herbal medicine

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Bioassay coupled fractionation for development of anti-HCV herbal medicine
	Chen, SR; Lin, L.; Wang, Y. T.; Wang, Y.
	2015-08-01
Source Publication	14th Meeting of Consortium for Globalization of Chinese Medicine
Abstract	Hepatitis C virus currently infects 170 million people worldwide and causes a high rate of chronic infection (>75%), which progresses into cirrhosis and hepatocellular carcinoma. The current treatment for HCV is direct anti-viral agents, and/or peginterferon alpha plus ribavirin. The sustained viral response varies in different cohort, and the high cost limits the broad use of current treatment. Therefore, alternative treatment for HCV is still needed. Herbal medicines regulating and notifying blood and target against the “liver” channel are of our primary focus to investigate on the anti-HCV activity. Crude ethanol and water extracts from the rhizomes of different herbal medicine were obtained by using different chromatography methods, and analyzed for the anti-HCV activity in HCV genotype 1b replicon cells. Among the examined herbal medicines, ethanol extracts of Spatholobi Caulis and Crataegus pinnatifida Bge exhibited potent anti-HCV activity. Extracts from Fructus Lycii, Radix Astragali, Rubus Chingii Hu, Flos Chrysanthemi Indici, Cassia obtusifolia, Lonicera japonica (L. japonica) Thunb, Forsythia suspensa (Thunb.) Vahl, Poria cocos (Schw.) Wolf, Carthamus tinctorius L, Leonurus japonicus Houtt failed to show activity in the HCV replicon cells. Further study will be needed to confirm the anti-HCV activity. These results suggested that in vitro bioassay coupled fraction methods could be used as the prior first step in the development of novel activities of herbal medicine.
Keyword	hepatitis c virus herbal medicine bioassay coupled fractionation
Language	英語English
The Source to Article	PB_Publication
PUB ID	15352
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Wang, Y.
Recommended Citation GB/T 7714	Chen, SR,Lin, L.,Wang, Y. T.,et al. Bioassay coupled fractionation for development of anti-HCV herbal medicine[C], 2015.
APA	Chen, SR., Lin, L.., Wang, Y. T.., & Wang, Y. (2015). Bioassay coupled fractionation for development of anti-HCV herbal medicine. 14th Meeting of Consortium for Globalization of Chinese Medicine.

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