Are spores dormant? A perspective from the transcription level.

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Are spores dormant? A perspective from the transcription level.
	Wang, F.; Dong, L.; Wong, K. H.
	2017-06-21
Source Publication	4th Macau Symposium on Biomedical Sciences 2017
Abstract	Fungal spores are specialized reproductive cells of filamentous fungi with properties for dispersal and survival under harsh conditions and over a long period of time. Previous studies have detected mRNA transcripts in resting spores of some filamentous fungi, but it is not clear whether the transcripts were carried over from conidiation or were actively synthesized in spores. In this work, we aim to find out 1) whether resting spores of Aspergillus nidulans have any active transcription, 2) whether spores can respond to stresses during dormancy or behave differently during germination. To address these, we have used Chromatin Immuno-precipitation followed by Next Generation Sequencing (ChIPseq) against RNA polymerase II (Pol II) to map active transcription events in spores kept under different conditions and during germination. Our preliminary results show that Pol II is engaged on many genes genome-wide in spores. GO analysis shows that these genes are enriched with stress response, ATP generation and the conidiation process. Unexpectedly, we found a significant level of Pol II accumulation near the 5’ end of many genes in resting spores but absent in mycelia, which is similar to the promoter-proximal pausing phenomenon in higher eukaryotes. Moreover, we also compared the active transcription in two other fungal pathogens—A. fumigatus and P. marneffei, we speculate that active transcription in spores is ubiquitous and Pol II paused to specific promoters such to reduce the steps and energy needed by activation of genes for growth and survival. Therefore, this Pol II activation and “pausing” phenomenon might have important implications in the physiology and durability of spores.
Keyword	spore transcription
Language	英語English
The Source to Article	PB_Publication
PUB ID	32323
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Wang, F.,Dong, L.,Wong, K. H.. Are spores dormant? A perspective from the transcription level.[C], 2017.
APA	Wang, F.., Dong, L.., & Wong, K. H. (2017). Are spores dormant? A perspective from the transcription level.. 4th Macau Symposium on Biomedical Sciences 2017.

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