Systemic Investigation of Global Transcription Regulation network of gluconeogenesis

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Systemic Investigation of Global Transcription Regulation network of gluconeogenesis
	Dong, L.; Chen, Y; Tan, K.; Wong, K. H.
	2017-06-21
Source Publication	4th Macau Symposium on Biomedical Sciences 2017
Abstract	Carbon metabolism is a central process for all living organisms. The reversible Glycolysis and Gluconeogenesis pathways must be precisely regulated to maintain cellular homeostasis and make best use of nutrient resources. In Aspergillus nidulans, a model organism for many industrially, biotechnologically and medically important filamentous fungi, the two pathways are transcriptionally regulated by at least four transcription factors AcuK, AcuM, FacB and CreA. However, how these four factors coordinately control global gene expression for the utilization of different carbon resources and associating biological processes is not clear. We generated transcriptome datasets associated with the four transcription factors on three carbon growth conditions and performed ChromHMM to identify the coordination of the above four transcription factors on genome scale, and correlated different TF coordination with different chromatin states on the corresponding conditions to study the effect of the four TF combinations. By comparing gene ontology processes of their target genes, our results suggest that the transcription factors positively and negatively work closely together to control many different physiological processes. After that, we calculated biological processes’ relationships from 96 gene expression profiles in order to understand how the four transcription factors regulate multiple biological processes simultaneously. Furthermore, we performed comparative genomics study over filamentous and yeast fungal species and found that the carbon regulators were adopted by the filamentous fungi to control filamentous-specific biological processes presumably during the separation of the two morphologically distinct groups of species. We are in the process of constructing and comparing gene regulatory networks in A. nidulans and S. cerevisiae for understanding transcription regulation evolution. From all the works, we aim to better understand the transcription regulatory mechanisms underlying carbon source utilization in fungi.
Keyword	fungi gluconeogenesis transcription
Language	英語English
The Source to Article	PB_Publication
PUB ID	32232
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Dong, L.,Chen, Y,Tan, K.,et al. Systemic Investigation of Global Transcription Regulation network of gluconeogenesis[C], 2017.
APA	Dong, L.., Chen, Y., Tan, K.., & Wong, K. H. (2017). Systemic Investigation of Global Transcription Regulation network of gluconeogenesis. 4th Macau Symposium on Biomedical Sciences 2017.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Affiliation No.
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh