Genome-wide analysis of the GATA transcription factor AreA in Aspergillus nidulans.

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	Genome-wide analysis of the GATA transcription factor AreA in Aspergillus nidulans.
	Li, A.; Miao, Z.; Downes, D. J.; Busot, G. Y.; Todd, R. B.; Wong, K. H.
	2017-03-14
Source Publication	29th Fungal Genetics Conference
Abstract	In Aspergillus nidulans, nitrogen utilization is precisely regulated at the gene expression level. Expression of genes encoding enzymes to utilize alternative nitrogen sources or to scavenge nitrogen compounds during nitrogen starvation is dependent on the GATA transcription factor AreA. In general for typical nitrogen metabolic genes, AreA-dependent activation is minimal during nitrogen sufficiency, increased when nitrogen is limiting, and strongest during nitrogen starvation. Although many AreA target genes are known, the genome-wide targets of AreA and AreA DNA binding properties remain unknown. To address this, we profiled AreA DNA binding under nitrogen sufficiency, nitrogen limitation and nitrogen starvation via Chromatin Immuno-precipitation followed by Sequencing (ChIP-seq) and identified more than 1,600 promoters bound by AreA. Interestingly, AreA binds to more than 400 promoters under nitrogen sufficiency, when its protein level in the nucleus is low. Expression analysis shows that less than 5% of these targets are affected by areA deletion. In contrast, under nitrogen starvation expression of more than 40% of AreA bound genes is significantly altered by areA deletion. These results indicate that AreA binds to many target promoters poised to activate upon appropriate signaling. Comparisons of targets among the different nitrogen conditions revealed many (~200) common binding sites as well as nitrogen source-specific AreA binding events, indicating that AreA targets different promoters according to nitrogen sources and availability. De novo motif discovery analysis showed that besides “GATA”, several other motifs are also enriched among AreA bound regions, consistent with cooperation of AreA with pathway-specific transcription factors. In addition to nitrogen metabolism, Gene Ontology analysis of AreA target genes suggests roles in development and stress response. Moreover, our data also provides compelling evidence for positive feedback control of AreA on the co-repressor NmrA that negatively regulates its function. Therefore, this work not only reveals the genome-wide functions of AreA, but also uncovers a new regulatory loop for nitrogen metabolism repression in A. nidulans.
Keyword	AreA transcription Aspergillus
Language	英語English
The Source to Article	PB_Publication
PUB ID	32196
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Li, A.,Miao, Z.,Downes, D. J.,et al. Genome-wide analysis of the GATA transcription factor AreA in Aspergillus nidulans.[C], 2017.
APA	Li, A.., Miao, Z.., Downes, D. J.., Busot, G. Y.., Todd, R. B.., & Wong, K. H. (2017). Genome-wide analysis of the GATA transcription factor AreA in Aspergillus nidulans.. 29th Fungal Genetics Conference.

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