CgXbp1 modulates temporal transcriptional dynamics in human fungal pathogen Candida glabrata during interaction with host macrophages.

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	CgXbp1 modulates temporal transcriptional dynamics in human fungal pathogen Candida glabrata during interaction with host macrophages.
	Rai, M. N.; Parsania, C.; Rai, R.; Wong, K. H.
	2017-03-14
Source Publication	29th Fungal Genetics Conference
Abstract	Candida glabrata, an opportunistic human fungal pathogen in immunocompromised patients, remodels its carbon metabolism to survive in nutrient-deprived intracellular macrophage microenvironment. Here, we have identified a transcription factor, CgXbp1, required for remodeling carbon metabolic pathways in C. glabrata upon exposure to host macrophages. Employing the next generation sequencing approaches, we showed that Cgxbp1Δ mutant exhibited constitutively active fatty acid catabolic and proteolytic pathways even in the presence of sufficient carbohydrate in the growth medium, suggesting that carbon metabolic processes are deregulated upon CgXBP1 deletion. Next, we demonstrated that CgXbp1 is essential for arresting the cells in G1 phase of cell cycle during carbon starvation. Global gene expression analysis on Cgxbp1Δ mutant revealed that genes associated with GO terms – mitochondrion organization, mitochondrial transport and mitochondrial translation were highly enriched among down-regulated genes, indicating that CgXbp1 may have pivotal roles in cellular energy homeostasis maintenance. Comparison of the transcriptional profiles of macrophage-engulfed wild-type and Cgxbp1Δ mutant cells revealed that unlike wild-type cells, Cgxbp1Δ mutant was unable to repress genes associated with GO terms - Translation, Ribosome biogenesis and RNA metabolic processes during early stage of macrophage infection. Overall, our gene expression data indicated that CgXbp1 not only is central in carbon metabolic remodeling, but also affects global transcriptional response of C. glabrata cells in host macrophages.
Keyword	Candida fungal infection
Language	英語English
The Source to Article	PB_Publication
PUB ID	32155
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Rai, M. N.,Parsania, C.,Rai, R.,et al. CgXbp1 modulates temporal transcriptional dynamics in human fungal pathogen Candida glabrata during interaction with host macrophages.[C], 2017.
APA	Rai, M. N.., Parsania, C.., Rai, R.., & Wong, K. H. (2017). CgXbp1 modulates temporal transcriptional dynamics in human fungal pathogen Candida glabrata during interaction with host macrophages.. 29th Fungal Genetics Conference.

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