| Status | 已發表Published |
| Pan-genomic analysis of TamA in Aspergillus nidulans: the regulatory network of a dual function Zn(II)2Cys6 transcription factor. | |
Downes, D.; Wong, K. H.  ; Todd, R.
| |
| 2015-03-17 | |
| Source Publication | 28th Fungal Genetics Conference
 |
| Abstract | Zn(II)2Cys6 zinc binuclear cluster proteins represent the largest family of transcription factors in fungi and regulate genes of diverse pathways, including primary and secondary metabolism, and development. For Zn(II)2Cys6 proteins, and indeed most transcription factors, the DNA binding domain is considered essential for function. However proteins in the emerging class of dual function transcription factors operate by either direct DNA binding or as protein-binding co-activators and co-repressors (non-DNA-binding factors). The switch between functions can be determined by promoter context and cell type. Dual function has been reported in different cell types for the Hairy repressor in Drosophilia, Xenopus and zebrafish as well as mouse SCL/TAL-1. We recently reported the first example of a fungal dual function transcription factor, the Aspergillus nidulans Zn(II)2Cys6 transcription factor TamA. TamA is unique in that dual function occurs in the same cell type, where it binds the gdhA promoter directly while acting as a co-activator of the global nitrogen GATA transcription factor AreA at amdS and fmdS. To determine the extent of the TamA dual function regulatory network we have taken a pan-genomic approach. Using FLAG-epitope-tagged TamA (TamAFLAG) for ChIP-seq, we have identified novel targets of TamA, including genes involved in primary metabolism, secondary metabolism and siderophore biosynthesis. This analysis has indicated that TamA target sites lack sequence conformity. Therefore TamA may act via multiple DNA-binding sequences, potentially on the basis of interaction with novel proteins. To determine the TamA activator and co-activator regulons we characterized the effect of TamA on global gene expression using stranded RNA-seq in tamA∆ and tamAC90L loss-of-DNA binding mutants. The dual functions of transcription factors such as TamA provide an additional level of combinatorial control to mediate gene-specific expression. Analysis of the TamA regulatory network at a pan-genomic level provides insights into how dual function transcription factors facilitate promoter plasticity and act as a vehicle for adaptive evolution. |
| Keyword | Aspergillus nidulans TamA Transcription factor |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 13868 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences |
| Corresponding Author | Wong, K. H.; Todd, R. |
| Recommended Citation GB/T 7714 | Downes, D.,Wong, K. H.,Todd, R.. Pan-genomic analysis of TamA in Aspergillus nidulans: the regulatory network of a dual function Zn(II)2Cys6 transcription factor.[C], 2015. |
| APA | Downes, D.., Wong, K. H.., & Todd, R. (2015). Pan-genomic analysis of TamA in Aspergillus nidulans: the regulatory network of a dual function Zn(II)2Cys6 transcription factor.. 28th Fungal Genetics Conference. |
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