| Status | 已發表Published |
| Studying the role of NF-κB and Bcl3 in zebrafish fin regeneration and bone development | |
Fan, C. ; Wang, V. Y.-F. 
| |
| 2021-07-23 | |
| Source Publication | The 7th Macau Symposium on Biomedical Sciences Abstract Book
 |
| Abstract | Osteoporosis is a metabolic bone disease caused by an altered balance between bone anabolism and catabolism. This dysregulated balance is responsible for fragile bones that fracture easily after minor falls. With an aging population, the incidence is rising, making it especially important to study the disease in depth. Osteoclasts are responsible for aged bone resorption and osteoblasts are responsible for new bone formation. The resorption and formation are stable at physiological conditions. NF-κB is a pleiotropic transcription factor, that is essential for RANK (receptor activator of NF-κB)-expressing osteoclast precursors to differentiate into TRAP+ osteoclasts in response to RANKL (RANK ligand) and other osteoclastogenic cytokines. The nuclear factor-κB (NF-κB) transcription factor is a critical regulator of immediate responses to pathogens and also plays an important role in regulating cell proliferation and survival. In humans, there are five members of the NF-κB family (RelA, RelB, c-Rel, NF-κB1 and NF-κB2), and eight members of the IκB family (IκBα, IκBβ, IκBε, IκBγ, IκBδ, Bcl3, IκBζ, and IκBNS). In zebrafish, there are five members of the NF-κB family, while only six members of the IκB family have been found so far (IκBα, IκBβ, IκBε, IκBγ, IκBδ, and Bcl3). By sequence alignment, these zebrafish members were found to be about 50 percent homologous to human. zebrafish are vertebrates and show strong similarities in their skeletal physiology to mammals, combined with the zebrafish's genetic tractability, the structure of the modified genome can be injected directly into the embryo at the single-cell stage. This allowed the generation of transgenic lines that allow dynamic imaging of all the cells of the developing skeletal system in live larvae, making zebrafish an important new animal model for studying skeletal development. The oncogenic protein Bcl3 is a nuclear IκB that can activate or represses gene transcription through binding with the NF-κB proteins p52. Bcl3 and p52 are important regulatory factors in the NF-κB signaling pathway, so we wanted to build Bcl3-/-, p52-/- and Bcl3-/- p52-/- knockout model in zebrafish to study the important roles of Bcl3 and p52 in fin regeneration and bone development of zebrafish. |
| Keyword | Bone development Zebrafish NF-kB |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 58701 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES |
| Corresponding Author | Wang, V. Y.-F. |
| Recommended Citation GB/T 7714 | Fan, C.,Wang, V. Y.-F.. Studying the role of NF-κB and Bcl3 in zebrafish fin regeneration and bone development[C], 2021. |
| APA | Fan, C.., & Wang, V. Y.-F. (2021). Studying the role of NF-κB and Bcl3 in zebrafish fin regeneration and bone development. The 7th Macau Symposium on Biomedical Sciences Abstract Book. |
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