| Residential College | false |
| Status | 已發表Published |
| Identification of a novel HLA-A2-restrictive CTL epitope of an ovary cancer-associated antigen OVA66 | |
| Jin S.; Wang Y.; Wang S.J.; Zhang H.Z.; Li M.X.; Chen Y.; Ge H.L. | |
| 2005-03-01 | |
| Source Publication | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
 |
| ISSN | 10078738 |
| Volume | 21Issue:2 |
| Abstract | AIM: To identify a novel HLA-A2-restrictive CTL epitope of an ovary cancer-associated antigen OVA66. METHODS: Dendritic cells (DCs), induced from peripheral blood mononuclear cells(PBMCs) by cytokines, were confirmed by morphological observation and FACS. Mature DCs were pulsed with each of the two synthesized peptides which were selected as possible CTL epitopes by software analysis. The pulsed DCs were used to stimulate autologous CD8+ T cells from an HLA-A2+ healthy donor. One week later, the peptides-pulsed autologous PBMCs were used to stimulate the CD8+ T cells for another 3 times at weekly intervals. The stimulated CD8+ T cells were used as CTLs. The cytotoxicity of CTLs to target cells was detected by lactate dehydrogenase (LDH) release assay and the number of T cells secreting antigen-specific IFN-gamma in CTLs was analyzed by enzyme-linked immunospot assay (ELISPOT). RESULTS: The results of morphology observation and FACS indicated that mature DCs were induced from PBMCs. Of the two peptides, peptide L235(FLPDHINIV) induced peptide-specific CD8+ T cells that lysed HLA-A2+ T2 cells pulsed with L235 and OVA66+/HLA-A2+ SW480 cells. Compared with control peptide, L235 increased the number of IFN-gamma producing T cells. CONCLUSION: This novel OVA66-derived CTL epitope L235 can induce HLA-A2-restrictive CTL response, which lays the foundation for preparation of tumor-specific peptide vaccine. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | Shanghai Second Medical University |
| Recommended Citation GB/T 7714 | Jin S.,Wang Y.,Wang S.J.,et al. Identification of a novel HLA-A2-restrictive CTL epitope of an ovary cancer-associated antigen OVA66[J]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2005, 21(2). |
| APA | Jin S.., Wang Y.., Wang S.J.., Zhang H.Z.., Li M.X.., Chen Y.., & Ge H.L. (2005). Identification of a novel HLA-A2-restrictive CTL epitope of an ovary cancer-associated antigen OVA66. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 21(2). |
| MLA | Jin S.,et al."Identification of a novel HLA-A2-restrictive CTL epitope of an ovary cancer-associated antigen OVA66".Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 21.2(2005). |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment